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- W2383588766 abstract "Objective To generate a cell-based functional assay for human pituitary adenylate cyclase activating peptide Ⅰ receptor (PAC_1-R) agonist screening. Methods The human PAC_1-R cDNA was cloned into a mammalian expression vector pCDNA3.1 (hPAC_1R/pCDNA3.1). This cDNA plasmid was co-transfected with a reporter gene construct (SRE/3×MRE/CRE/VIP/Luci) in CHO cells. Stable cell line expressing the human PAC_1-R and the reporter gene was selected with G418. The assay condition was optimized with PAC_1-R ligand PACAP-27. Results We have generated a stable cell line and reliable functional assay for PAC_1-R agonist screening. Our results demonstrated that the Z’-factor value was more than 0.7. Conclusion This technology can be optimized for high throughput screening and successfully applied to identify agonist for PAC_1-R." @default.
- W2383588766 created "2016-06-24" @default.
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- W2383588766 date "2006-01-01" @default.
- W2383588766 modified "2023-09-25" @default.
- W2383588766 title "Establishment of a cell-based assay for PACAP I receptor agonist screening" @default.
- W2383588766 hasPublicationYear "2006" @default.
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