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- W2383642232 abstract "The use of small hairpin RNA(shRNA) to regulate gene expression is evolving into an increasingly powerful tool for gene function study in organisms.We designed shRNAs with stems of 21 bp,27 bp,and 29 bp targeting to the enhanced green fluorescent protein gene.The synthesized single strands were annealed and cloned into psiSTRIKE including U6 promoter.Interfering fragments had successfully been cloned into expression vector tested by enzyme digesting and sequencing.The work in this study will help us to value the silencing efficacy of different stem shRNAs in mouse cell and individual in future research." @default.
- W2383642232 created "2016-06-24" @default.
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- W2383642232 date "2010-01-01" @default.
- W2383642232 modified "2023-09-24" @default.
- W2383642232 title "Construction of shRNA Expression Vectors Embracing 21 bp,27 bp,or 29 bp Stem" @default.
- W2383642232 hasPublicationYear "2010" @default.
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