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- W2383670658 abstract "Connective tissue growth factor(CTGF)emerges in both physiological and pathological processes and may lead to fibrosis,which provides a new target for therapeutic intervention.A867bp cDNA fragment of human-CTGF was amplified from human placenta by RT-PCR and subcloned into the expression vector pET9a.Then it was trans-formed into E.coli strain BL21(DE3)pLsS.The truncated recombinant h-CTGF lacked N terminal61aa and mainly occured in inclusion bodys.rh-CTGF was isolated and purified and could not stimulate the proliferation of fibroblast.The strategy of CTGF expression in prokaryotic cells was discussed.All above will be useful for antibody preparation against CTGF and further research of CTGF expression regulation and anti-fibrosis intervention." @default.
- W2383670658 created "2016-06-24" @default.
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- W2383670658 date "2002-01-01" @default.
- W2383670658 modified "2023-09-26" @default.
- W2383670658 title "Expression of truncated recombinant human connective tissue growth factor in E.coli" @default.
- W2383670658 hasPublicationYear "2002" @default.
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