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- W2383698425 abstract "Objective To establish a method to detect Bordetella pertussis based on real-time fluorescent quantitative PCR.Methods The primers and Taqman probe were designed according to the repetitive insertion sequence IS481 of Bordetella pertussis.The DNA fragment of IS481 gene of Bordetella pertussis amplified by PCR was used as the template to develop the assay and standard curve.The sensitivity,specificity and reproducibility of the developed real-time quantitative PCR were tested.Results A fine linear relationship(r =0.998) between the threshold cycle(Ct) values of the developed standard curve and the copy number of template was observed.The sensitivity of the assay was 102 copies/μl and the coefficients of variation of intra and inter-assay were less than 4%.No amplication curve of other respiratory pathogens was found thus perfect specificity was shown.Conclusions A real-time fluorescent quantitative PCR for detecting the repetitive element IS481 was successfully developed,and the approach should be available for rapid diagnosis of Bordetella pertussis with good sensitivity,specificity and reproducibility." @default.
- W2383698425 created "2016-06-24" @default.
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- W2383698425 date "2010-01-01" @default.
- W2383698425 modified "2023-09-26" @default.
- W2383698425 title "Development and evaluation of real-time quantitative PCR assay for rapidly detecting Bordetella pertussis" @default.
- W2383698425 hasPublicationYear "2010" @default.
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