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- W2383708481 abstract "OBJECTIVE:To construct the lentivirus vector containing FUS1 gene and prepare lentivirus for high transfection.METHODS:The full length of FUS1 gene was amplified by the method of PCR technique from the total RNA of the umbilical mesenchymal stem cells.FUS1 gene was cloned into pSL6-IRES-EGFP vector and identified with PCR,digestion and sequencing.The recombinant plasmid of pSL6-FUS1-IRES-EGFP was packaged in 293T cells and the lentivirus was collected.The infection effect was observed by transfection into BEL7404 and DLD-1 cells.RESULTS:The plasmid of pSL6-FUS1-IRES-EGFP was obtained and identified by means of PCR,digestion,sequencing.The lentivirus can infect cells more than 90%.CONCLUSION:The recombinant lentivirus vector of FUS1 was successfully cloned and the preparation of lentivirus may high effectively transfect cells." @default.
- W2383708481 created "2016-06-24" @default.
- W2383708481 creator A5014131549 @default.
- W2383708481 date "2008-01-01" @default.
- W2383708481 modified "2023-09-23" @default.
- W2383708481 title "Construction of lentivirus vector containing FUS1 gene" @default.
- W2383708481 hasPublicationYear "2008" @default.
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