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- W2383720659 abstract "Objective To evaluate the effectiveness of 16S ribosomal RNA(16SrRNA) general primer in bacterial detection and to find a method that could detect the bacteria rapidly and accurately in platelet concentrates.Methods Sample Genomic DNA was extracted by molecular biology method.16SrRNA general primer and 16S—23S ribosomal RNA interval primer were used for PCR,and the PCR products were treated with enzyme HaeⅢ.We compared the enzyme treated fragments with frequent bacteria sheet to determine whether the sample was contaminated or not.Once the sample was contaminated,the contaminated strain had to been confirmed.Results All detection were finished within 4 hours with 16SrRNA general primer method.No bacterium was detected in platelet concentrates during the 24-hour preservation.While in platelet concentrates preserved for 48-hour,S.epiderimidis and B.subtilis were detected,and the results were confirmed through auto bacteria culture.Conclusion 16SrRNA general primer method is rapid and accurate in the bacterial detection of platelet concentrates,suggesting the reliability and clinical value." @default.
- W2383720659 created "2016-06-24" @default.
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- W2383720659 date "2008-01-01" @default.
- W2383720659 modified "2023-09-24" @default.
- W2383720659 title "Application of 16S ribosomal RNA general primer in the bacterial detection of platelet concentrates" @default.
- W2383720659 hasPublicationYear "2008" @default.
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