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- W2383725096 abstract "The embryo-derived calli from Lolium multiflorum Lam were transformed with Agrobactrium tumefaciens AGL1 harboring intron-DREB1A expression vector pBI121 containing ubiqutin promoter,nptⅡ marker gene and GUS gene.After infection and co-culture with AGL1,the embyogenic calli were selected with 50 mg/L paromomycine and plants regenerated from the resistant calli.All plants were selected further with 25 mg/L kanamycin,some of which remained green.The genome DNA of the resistant plants was checked with specific primers and probes from the DREB1A gene.The results of PCR and PCR-Southern blot assay indicated that the DREB1A gene was transferred into Lolium multiflorum Lam with a transformation frequency of 2.14%.It was found that the transformation efficiency can be efficiently improved using the following transformation conditions: Agrobacterium cell density of OD600≈2.0,incubation for 1 hour,coculture at 21 ℃ for 2 days and application of As in coculture." @default.
- W2383725096 created "2016-06-24" @default.
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- W2383725096 date "2008-01-01" @default.
- W2383725096 modified "2023-09-26" @default.
- W2383725096 title "Agrobactrium tumefaciens mediated DREB1A gene transfer to Lolium multiflorum and optimization of its genetic transformation" @default.
- W2383725096 hasPublicationYear "2008" @default.
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