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- W2383862019 abstract "The V and W genes of the virulent NDV strain GM and lentogenic strain LaSota were acquired by gene mutation technique of two times polymerase chain reaction(PCR).The four genes were coloned into vector pMD-18 Tand then were sub-coloned into vector pEGFP-N1.The reconstituted plasmid pEGFP-La V,pEGFP-La W,pEGFPGM V and pEGFP-GM W were established by sequences anlysis.Green fluorescent protein(GFP)were expressed when the four plasimds were transfected into DF-1 cells,the intensity of GFP increased as time went on,and the intensity was highest at 36 hafter the plasimds were transfected into DF-1 cells while the control cells did not express any GFP.The results showed that the fusion protein W and V could express after the plasimds pEGFP-La V,pEGFP-La W,pEGFP-GM V and pEGFP-GM W were transfected into DF-1 cells.It laid a good foundation for studying the function of NDV V and W protein." @default.
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- W2383862019 date "2013-01-01" @default.
- W2383862019 modified "2023-09-25" @default.
- W2383862019 title "Expression of Recombinant Newcastle Disease Virus V and W Genes in DF-1 Cells" @default.
- W2383862019 hasPublicationYear "2013" @default.
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