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- W2383917145 abstract "Using prokaryotic expressed GST-BoPrP (23--242) fusion protein as antigen, BALB/C mice were immunized to prepare the specific antiserum against the yak protein protein. By Western blotting and indirect ELISA detecting, the antiserum could react with the yak recombinant mature PrP (23--242) and the extraction of bovine brain tissue, respectively. After digestion of the antigens by proteinase K, the immune reaction disappeared. However the antiserum could not be reacted with the GST protein and the full lysate of E.coliBL21 (DE3). The results indicated that the antiserum was the one against the yak recombinant mature PrP (23--242) with its titer higher than 1:12800, and it could detect the native prion proteins from the brain tissue homogenate of cattle. The GST-PrP (23--242) fusion protein could efficiently stimulate the immunized animals to produce the PrP-specified antibody, and the antiserum could be used for detecting the native prion proteins." @default.
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- W2383917145 date "2004-12-01" @default.
- W2383917145 modified "2023-09-23" @default.
- W2383917145 title "Purification and activity determination of recombinant prion protein of yak expressed in prokaryotic cell" @default.
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