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- W2383966389 abstract "Genetic transformation has been achieved by incubating protoplasts from Chaetomium globosum with plasmid PCB1003. The efficiency was 1 2 transformants/μg vector DNA. Protoplasts were produced by treating 14 hour old mycelium of Chaetomium globosum with 10 mg/mL lysozyme. Chaetomium globosum growth was inhibited completely when the medium contained 150 μg/mL hygromycin B. Integration of hph gene was verified by PCR and PCR product hybridization analysis. There was no evidence of mitotic of hygromycin B resistance in the transformants." @default.
- W2383966389 created "2016-06-24" @default.
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- W2383966389 date "2001-01-01" @default.
- W2383966389 modified "2023-09-25" @default.
- W2383966389 title "Transformation of Chaetomium globosum with hygromycin B resistance plasmids pCB1003" @default.
- W2383966389 hasPublicationYear "2001" @default.
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