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- W2383977797 abstract "Objective:To prepare and characterize the monoclonal antibodies to recombinant Adiponectin(APN) for further investigating its potential action on obesity,therosclerosis,diabetic angiopathy and fetal growth.Methods:The human APN was synthesized by the FMP solid-phase organic chemical method and purified by HPLC,then the purified recombinant APN was immunized to synthesized the BALB/c mice to prepare the McAb.The antibody titers,specificity,subclass and antigenic determinants of the McAb were identified and detected.Results:After cell fusion and subcloning,the clonal rate was 85.4%,the positive rate was 3.2%,the antibody titers in the ascites fluid was 1.0×107 and McAbs had no cross reaction with endoplasm,leptin,BSA and HAS.Eight hybridoma cell lines were obtained:A1~8,among them,A2,A3,A4,A6,A8 belonging to the subtypes of IgG2a,whereas A1,A5,A7 belonging to IgG2b.The results of cotitration and double antibody competitive test showed that A1 recognized one antigenic determinant,while A2 and A3 recognized another antigenic determinant.Conclusion:The preparation of the APN-McAb may provide the important foundation for further studying the multi-biological properties and high efficiency of the APN." @default.
- W2383977797 created "2016-06-24" @default.
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- W2383977797 date "2008-01-01" @default.
- W2383977797 modified "2023-09-24" @default.
- W2383977797 title "Preparation and Analysis of Immunologic Identification of the Monoclonal Antibodies to Human Adiponectin" @default.
- W2383977797 hasPublicationYear "2008" @default.
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