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- W2384014247 abstract "Objective To explore the effects of biotin dextran amine (BDA) as a nerve tracer in rat corti- cospinal tract.Methods Adult SD rats were used in this experiment and 15% BDA was injected slowly into the left sensory and motor cortex with a mini-syringe through two round-shape bone windows with 3mm diameter, formed by a dental drill.Three spots were chosen for injection within each bone window in different layers,with the total volume 108μl.After survival for 14 days,brains and spinal cords were removed and stored in a freezer.Sec- tions were made in a cryostat and transportation of BDA after absorption by axons were observed under a fluores- cence microscope.Results The BDA injected into the left cortex is taken by the axon,and the pyramidal cells and the axon in the left cerebral cortex appeard the widespread fluorescence development,with the time going on,the BDA taken by the cerebral cortex axon transfers to the midbrain and cervical enlargement along the CST with long distance,and structures of axon and nodes of ranvier could be observed clearly with a 200×magnification. Conclusion Injecting the BDA into the cerebral cortex can trace the corticospinal tract effectively.It is biologically stable and can be transported for a long distance.The experiment using spontaneous fluorescence is simple and pro- vides a reliable method for the morphological study of corticospinal tract of rats." @default.
- W2384014247 created "2016-06-24" @default.
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- W2384014247 date "2007-01-01" @default.
- W2384014247 modified "2023-09-25" @default.
- W2384014247 title "Effect of biotinylated dextran amine as a nerve tracer in rat corticospinal tract" @default.
- W2384014247 hasPublicationYear "2007" @default.
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