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- W2384014686 abstract "OBJECTIVE:To extract the genomic DNA from preserved specimen and apply it for molecular research.METHODS:After being taken off paraffin wax with xylene from paraffin-embedded lung cancer tissue, first the tissue was infused into PBS buffer for 4-8 hrs in water bath, the temperature was 60℃, then handled with different concentration of alcohol.RESULTS:Long DNA fragment was acquired by this method, and higher yields of DNA were got at pH 7.5-8.5.The PCR products amounted to 675 bps.CONCLUSIONS:Long DNA fragment can be acquired from the PBS buffer (pH 7.5-8.5) pretreatment preserved specimen.It is important for molecular research of sample DNA." @default.
- W2384014686 created "2016-06-24" @default.
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- W2384014686 date "2004-01-01" @default.
- W2384014686 modified "2023-09-26" @default.
- W2384014686 title "DNA extraction from paraffin-embedded lung cancer tissue and PCR" @default.
- W2384014686 hasPublicationYear "2004" @default.
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