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- W2384093577 abstract "To study the effect of hyperglycemia(HG) on cultured and hypoxic neurons and its possible mechanism related to Ca 2+ ,a hypoxic model in vitro was used.The activity of cells was measured by MTT assay and trypan blue straining when the final concentrations of glucose in nutrient medium were 22 7(control group,CG),30,40,50,60(hyperglycemia group,HGG)mmol/L, respectively.Intracellular free Ca 2+ concentration ([Ca 2+ ] i) in neurons was measured by RF 5000 double wave length fluorospectrophotometer,with Fura 2/Am as Ca 2+ fluorescent indicator.We found that cultured and hypoxic neurons were damaged only in HGG,and the resting [Ca 2+ ] i of neurons in Ca 2+ contain and Ca 2+ free medium were significantly higher than that of CG;but in Ca 2+ contain medium,the HG could not ease the inflow of Ca 2+ by irritating neurons with KCl and Glu ( P 0 05);and the rate of increase of [Ca 2+ ] i with adding CaCl 2 had no significant difference between CG and HGG in Ca 2+ free medium (P0 05).So it could be probably suggested that HG might contribute to Ca 2+ overload by promoting Ca 2+ out flowing from intracellular Ca 2+ pools." @default.
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- W2384093577 date "2000-01-01" @default.
- W2384093577 modified "2023-09-25" @default.
- W2384093577 title "Study of the effect of hyperglycemia on hypoxic neurons and its Ca~(2+) related mechanism" @default.
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