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- W2384105220 abstract "Objective To clone,express,and identify the the gene of Cor h 1 from Corylus heterophylla.Methods The Cor h 1 gene was amplified by PCR and cloned into pMD18-T for sequencing.Then the interesting gene was subcloned into pET-28a for expression.Results The length of gene was 486 bp,coding 161 amino acids.The E-coli strain could express a recombinant protein with a molecular weight of 17 600.Conclusion The gene of Cor h 1 is successfully cloned and expressed in this study,and the recombinant protein possesses good IgE-binding capacity." @default.
- W2384105220 created "2016-06-24" @default.
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- W2384105220 date "2009-01-01" @default.
- W2384105220 modified "2023-09-24" @default.
- W2384105220 title "Cloning , expression , and identif ication the gene of Cor h 1 from Corylus Heterophylla" @default.
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