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- W2384117732 abstract "Chloroperoxidase(CPO)from Caldariomyces fumago is a highly versatile oxidase.But its application is limited,because of its low accumulated concentration in the biological synthesis,poor dynamic stability,low recovery of high-purity enzyme in purification process and high price.A novel very efficient method has been developed.At first,CPO was precipitated with hydrophilic polymer(PEG 6000)in the high-saturated solution of aluminum sulfate.And then it was extracted by an aqueous two-phase system of PEG/ammonium sulfate.At last,the extracted CPO was purified by Sephadex G-75 chromatographic system.The results showed that:60% saturated(NH4)2SO4 solution could make PEG 6000 precipitate 95% chloroperoxidase.In the aqueous two-phase system CPO was prior to dissolve in bottom phase,its partition coefficient k was lower than 0.228,its yield about 65.2%,its purification was 7.4 times.Finally,the purity of CPO was slightly higher after chromatographic fractionation.The method was also provide a new method for extracting efficiently other trace biological molecules." @default.
- W2384117732 created "2016-06-24" @default.
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- W2384117732 date "2010-01-01" @default.
- W2384117732 modified "2023-09-27" @default.
- W2384117732 title "Study on the conditions for purification of chloroperoxidase by aqueous two-phase system of PEG co-precipitation and resolution" @default.
- W2384117732 hasPublicationYear "2010" @default.
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