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- W2384117887 abstract "Objective To study the effect of RhoB on the proliferation of gastric cancer cell line SGC7901. Methods The inducible expression vector pGene/V5-His-RhoB containing the full length RhoB cDNA was constructed and transfected into gastric cancer cell SGC7901/pSwitch by lipofectamine, which was derived from SGC7901 after transfected with a regulatory plasmid pSwitch. The expression of RhoB in the screened stable cell lines were detected by Western blot after the induction of mifepristone for 0, 3, 6, 12, 24 hours. The effect of RhoB on the proliferation on gastric cancer cell line SGC7901 was evaluated by MTT assay. The apoptotic index was tested by Hoechst 33258 staining and FCM.Results The inducible expression vector pGene/V5-His-RhoB was constructed and confirmed by enzymatic digestion and DNA sequencing. After transfected into SGC7901 cells, RhoB protein expression, after mifepristone induction, was in a time dependence manner in 24 hours. MTT assay showed that RhoB suppressed growth of gastric cancer cells remarkably. Detection of apoptosis showed that RhoB enhanced apoptosis of the gastric cancer cells. Conclusion RhoB plays an important tumor-suppressive role in gastric cancer." @default.
- W2384117887 created "2016-06-24" @default.
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- W2384117887 date "2005-01-01" @default.
- W2384117887 modified "2023-09-24" @default.
- W2384117887 title "Suppressive effect of RhoB on gastric cancer cell line SGC7901." @default.
- W2384117887 hasPublicationYear "2005" @default.
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