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- W2384127613 abstract "To construct B16F10 tumor vaccine that express the IL-21 and the 6 kD early secreted antigenic target(ESAT-6) anchored by glycosylated phosphatidylinositol(GPI) on cell membrane and to identify its biological activity,plasmid recombination method based on overlap extension PCR was used to construct the eukaryotic expression vector for pIRES-ESAT-6-gpi/IL-21,then the melanoma B16F10 cells were transfected with the recombinant plasmid.The expressions of the fused protein and IL-21 were determined by RT-PCR,immunofluorescence,FCM,Western blot assay,respectively.Mouse splenocytes were cultivated with supernatant from the tumor vaccine cells to detect the biological activity of IL-21 secreted by tumor vaccine cells.It was demonstrated that the ESAT-6 target molecule was expressed on the surface of B16F10-ESAT-6-gpi/IL-21 tumor vaccine cells,and the exogenous gene did not affect the proliferation of vaccine cells.Furthermore,the IL-21 secreted from vaccine cells has biological activity.The stable transfected B16F10-ESAT-6-gpi/IL-21 tumor vaccine cell line was successfully constructed and the vaccine could be used for further anti-tumor research." @default.
- W2384127613 created "2016-06-24" @default.
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- W2384127613 date "2011-01-01" @default.
- W2384127613 modified "2023-09-26" @default.
- W2384127613 title "Construction of B16F10 tumor vaccine expressing ESAT-6-gpi and IL-21 and identification of its biological activity" @default.
- W2384127613 hasPublicationYear "2011" @default.
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