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- W2384140306 abstract "According to the conserved region of the major capsid protein gene of orange-spotted grouper(Epinephelus coioids) nervous necrosis virus(OGNNV),a pair of primers was designed.Using the primers,a RT-PCR product of 426 bp was cloned from the RNA template extracted from orange-spotted grouper infected by OGNNV.The RT-PCR product labeled by digoxigenin(DIG) was used for the non-radioactive nucleic acid probe.A group of orange-spotted groupers were artificialiy infected by injecting infected tissue filtrates and then the eyes and brain of moribund fish were fixed,and OGNNV was detected by in situ hybridization(ISH).The experiment showed that in situ hybridization is very sensitive and special,So we can use this method to detect whether grouper is infected with OGNNV or not.It can be used in inspecting and preventing the disease of VNN.The eyes and brains from diseased orange-spotted grouper were also detected by hematoxylinand eosin(HE) to compare with the result of in situ hybridization.We can look into the putrescence portion of the cells to make sure that the result is credible and nice.The established ISH for detecting OGNNV is highly sensitive,special and easily manipulated,and helpful to studies on virus location in tissue and pathogenesis." @default.
- W2384140306 created "2016-06-24" @default.
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- W2384140306 date "2008-01-01" @default.
- W2384140306 modified "2023-09-24" @default.
- W2384140306 title "Application of in situ hybridization in testing orange-sported grouper nervous necrosis virus" @default.
- W2384140306 hasPublicationYear "2008" @default.
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