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- W2384152490 abstract "Objective:To develop and validate a sensitive and specific high performance liquid chromatography(HPLC)method for the determination of hesperidin.Method:Hesperidin was analyzed by a Hypersil ODS2 column using 16 ∶84(v/v)acetonitrile/water as eluent which containede 0.06% acetic acid.Results:The method for analyzing hesperidin was proved to be accurate and precise with the linearity range being of 3.00 to 30.00mg/L.The linear correlation coefficient r≥0.9997 and the limitation of the detection for the method was 6.00ng.The precision experiment,carried out by injecting the same hesperidin sample for six times,showed that the relative standard deviation(RSD)for the peak areas was 0.08%.The recovery rate was 98.80%～103.12%.The stability experiment indicated that the degradation rates of hesperidin were all less than 1.70% and 1.59% within 8 days when stored at 4℃ and 30℃,respectively.Conclusion:The method established was simple,accurate and sensitive.It is suitable to analyze hesperidin using by HPLC method." @default.
- W2384152490 created "2016-06-24" @default.
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- W2384152490 date "2011-01-01" @default.
- W2384152490 modified "2023-09-23" @default.
- W2384152490 title "Study on quantitative determination of hesperidin by high performance liquid chromatography" @default.
- W2384152490 hasPublicationYear "2011" @default.
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