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- W2384152809 abstract "Saccharomyces cerevisiae is an important strain of glutathione production in fermentation. The screening of high glutathione-producing mutant and optimizing its culture conditions were studied. A strain of Saccharomyces cerevisiae G796-2 was isolated from 8 yeast strains for its higher intracellular glutathione content, and then the strain was mutagenized by ultraviolet and ethionine. A new mutant Saccharomyces cerevisiae SE-2 was isolated after two generation mutation whose intracellular glutathione content reached 2.2%, it is 62.9% higher than its parent in flask experiment. Results show that its high glutathione-producing ability can be inherited after several generation cultures. The orthogonal method results of the mutant culture conditions and the experimental results of two carbon sources test are as following: the optimal glucose concentration is 6%, the optimal rate of glucose/molasses is 3.5:1 and the optimal cysteine concentration is 5mmol?L?1. The glutathione yield of SE-2 was 180mg?L?1after cultivating 39 hours under the optimum conditions , it was 81.2% higher than that of G796-2." @default.
- W2384152809 created "2016-06-24" @default.
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- W2384152809 date "2005-01-01" @default.
- W2384152809 modified "2023-09-22" @default.
- W2384152809 title "Screening of Saccharomyces cerevisiae for Glutathione Over-production and Study of Culture Conditions" @default.
- W2384152809 hasPublicationYear "2005" @default.
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