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- W2384166875 abstract "Objective To develop a fluorescent microsphere-based lateral flow assay for the quantitative detection of casein in milk.Methods Casein was used as the antigen to immunize the New Zealand rabbits to make the corresponding polyclonal antibody against the antigen,and EDC mediated method was used to couple the purified polyclonal antibody with fluorescent microspheres.The filter pad,sample pad,conjugate pad,NC membrane,and absorption pad were assembled to develop the fluorescent microsphere-based lateral flow assay for the quantitative detection of casein in milk.Results The results could be obtained within 25 min without sophisticated equipment,and the limit of detection of the test strip was 100 ng/mL.The developed assay was highly specific to the casein protein as a result of that it had no cross-reactivity with other non-target proteins,such as BSA and OVA.The recovery rates of the intra-assay at casein concentrations of 100.0,500.0,and 1000.0 ng/mL were(89.03±5.2)%,(93.47±6.9)% and(91.2±7.8)%,respectively,while the inter-assay were(87.69±6.2)%,(92.73±8.3)% and(89.82±8.5)%,respectively.Conclusion The fluorescent microsphere-based lateral flow assay for the quantitative detection of casein in milk with the advantages of rapid,convenient,and high sensitivity was established preliminarily." @default.
- W2384166875 created "2016-06-24" @default.
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- W2384166875 date "2014-01-01" @default.
- W2384166875 modified "2023-09-25" @default.
- W2384166875 title "Fluorescent microsphere-based lateral flow assay for the quantitative detection of casein in milk" @default.
- W2384166875 hasPublicationYear "2014" @default.
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