FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2384175028> ?p ?o ?g. }

• W2384175028 abstract "Actinomycetes, as important sources of naturally occurred antibiotics, are strains for discovery of natural anti-infective agents. In order to isolate and purify the endophytic actinomycetes from mangrove plants in Beilun Estuary National Nature Reserve, Guangxi Zhuang Autonomous Region, explore their potential antimicrobial activities against pathogenic bacteria and fungi, and preliminarily clarify taxonomic status of bioactive strains, all 15 samples from different parts of 5 mangrove plants were cultured separately in 14 different liquid media for 4 weeks,and the enriched cultural media were then spreaded on the corresponding agar media with standard serial dilution plating method to isolate and purify the colonies. A total of 200 isolates were purified from samples including 62 strains from Avicennia marina, 43 strains from Kandelia candel,38 strains from Rhizophora stylosa, 29 strains from Clerodendrum inerme and 28 strains from Excoecaria agallocha, respectively. After removing the duplicate isolates by comparison of their cultural and morphological characterizations, 106 purified endophytic strains were tested for their antimicrobial activities against 6 strains of bacteria and 20 strains of Candida albicans by point planted method and disk diffusion method. A total of 52 isolates showed positive results and the positive rate was 49.1%. Among them,inhibitory rates against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Proteusbacillus vulgaris, Hemolytic streptococcus and Klebsiella pneumoniae were 16%, 15.1%, 4.7%, 7.5%, 11.3% and 3.8%,respectively; Inhibitory rates aganist drug- sensitive strains of Candida albicans and drug- resistant strains of Candida albicans were 16.0% and 29.2%, respectively. The bioactive strains were identified preliminarily as Streptomyces(21 strains), Nocardiopsis(26 strains), Pseudonocardia(2 strains), Agrococcus(2 strains) and Isoptericola(1 strain) by phylogenetic analysis based on 16 S r RNA gene sequences. Among them, the similarities of both strains UMBR1016 and UMBR1034 with Nocardiopsis alba DSM 43377 T were 98.27%and 98.37%, respectively; the similarity between strain UMBR 1008 and Nocardiopsis flavescens SA6 T, and between strain UMBR 1028 and Streptomyces pratensis ch24 T, was 98.36% and 98.49%, respectively. The results that similarities of 16 S r RNA gene sequences were less than 98.6% indicated 3 strains including UMBR1016, UMBR1034 and UMBR 1008 were potential new species of Nocardiopsis genus and UMBR1028 was potential new species of Streptomyces genus. The 38 and 35 isolates were found to show antimicrobial activity against at least one tested bacteria and one strain of Candida albicans, and the inhibitory rates were 35.8% and 33.0%, respectively. Abundant endophytic actinomycetes, including new species and potential antimicrobial activities found in the study, indicated that endophytic actinomycetes from mangrove plants in Beilun of Guangxi province could be a potential source for the discovery of new species and natural products and deserved further development in next step. The study will improve the conservation of mangrove plants in our country and further utilization of endophytic actinomycetes as important sources of drug discovery." @default.
• W2384175028 created "2016-06-24" @default.
• W2384175028 creator A5064967917 @default.
• W2384175028 date "2015-01-01" @default.
• W2384175028 modified "2023-09-26" @default.
• W2384175028 title "Isolation, Identification and Bioactivity of Endophytic Actinomycetes from Mangrove Plants in Beilun River" @default.
• W2384175028 hasPublicationYear "2015" @default.
• W2384175028 type Work @default.
• W2384175028 sameAs 2384175028 @default.
• W2384175028 citedByCount "0" @default.
• W2384175028 crossrefType "journal-article" @default.
• W2384175028 hasAuthorship W2384175028A5064967917 @default.
• W2384175028 hasConcept C145081991 @default.
• W2384175028 hasConcept C18903297 @default.
• W2384175028 hasConcept C2775947960 @default.
• W2384175028 hasConcept C2778660310 @default.
• W2384175028 hasConcept C2780717840 @default.
• W2384175028 hasConcept C2780917455 @default.
• W2384175028 hasConcept C4937899 @default.
• W2384175028 hasConcept C523546767 @default.
• W2384175028 hasConcept C54355233 @default.
• W2384175028 hasConcept C68874143 @default.
• W2384175028 hasConcept C86803240 @default.
• W2384175028 hasConcept C89423630 @default.
• W2384175028 hasConceptScore W2384175028C145081991 @default.
• W2384175028 hasConceptScore W2384175028C18903297 @default.
• W2384175028 hasConceptScore W2384175028C2775947960 @default.
• W2384175028 hasConceptScore W2384175028C2778660310 @default.
• W2384175028 hasConceptScore W2384175028C2780717840 @default.
• W2384175028 hasConceptScore W2384175028C2780917455 @default.
• W2384175028 hasConceptScore W2384175028C4937899 @default.
• W2384175028 hasConceptScore W2384175028C523546767 @default.
• W2384175028 hasConceptScore W2384175028C54355233 @default.
• W2384175028 hasConceptScore W2384175028C68874143 @default.
• W2384175028 hasConceptScore W2384175028C86803240 @default.
• W2384175028 hasConceptScore W2384175028C89423630 @default.
• W2384175028 hasLocation W23841750281 @default.
• W2384175028 hasOpenAccess W2384175028 @default.
• W2384175028 hasPrimaryLocation W23841750281 @default.
• W2384175028 hasRelatedWork W1579502513 @default.
• W2384175028 hasRelatedWork W2061196942 @default.
• W2384175028 hasRelatedWork W2118798086 @default.
• W2384175028 hasRelatedWork W2145374025 @default.
• W2384175028 hasRelatedWork W2314851037 @default.
• W2384175028 hasRelatedWork W2341971691 @default.
• W2384175028 hasRelatedWork W2347413650 @default.
• W2384175028 hasRelatedWork W2362089535 @default.
• W2384175028 hasRelatedWork W2362794322 @default.
• W2384175028 hasRelatedWork W2367587621 @default.
• W2384175028 hasRelatedWork W2372338497 @default.
• W2384175028 hasRelatedWork W2378599835 @default.
• W2384175028 hasRelatedWork W2380278187 @default.
• W2384175028 hasRelatedWork W2392730900 @default.
• W2384175028 hasRelatedWork W2558976935 @default.
• W2384175028 hasRelatedWork W2578264971 @default.
• W2384175028 hasRelatedWork W2589898265 @default.
• W2384175028 hasRelatedWork W2964966130 @default.
• W2384175028 hasRelatedWork W3031591448 @default.
• W2384175028 hasRelatedWork W46173500 @default.
• W2384175028 isParatext "false" @default.
• W2384175028 isRetracted "false" @default.
• W2384175028 magId "2384175028" @default.
• W2384175028 workType "article" @default.