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- W2384189192 abstract "Objective To investigate the more effective methods of isolation, culture of mesenchymal stem cell (MSCs), and examine the phenotype of MSCs in lab of Shenzhen Institute of Integrated Medicine of Shenzhen Second People's Hospital. Methods MSCs were separated from 3~4 weeks rats bone marrow by plastic adherence methods and purified by controlling the time of digestion combined with adhesion separation, and proliferated in culture flasks. Immunocytochemical method identified the MSCs active and phenotype. Results Primary cultured MSCs were oval, spindle-shaped or polygonal, and adhered to plastic surface within 24 h and reached 90% confluence within 7~8 days. After purification and proliferation, they were uniformly long spindle-shaped and passaged every seven days. The adhesion rate within 24 hours was 99%. Immunocytochemistry showed MSCs were positive for CD44, while negative for CD34. Conclusion A feasible method for the isolation, purification and pro1iferation of MSCs from rat bone marrow has been established. The cultured MSCs are active in high purity with stable biological character. The culture method combining adhesion separation and digestion control is simply performed in need of little condition, so it is one of the optimal methods for cell preparation and purification at present." @default.
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- W2384189192 date "2008-01-01" @default.
- W2384189192 modified "2023-09-23" @default.
- W2384189192 title "Isolation, Culture and Identification of Rat Mesenchymal Stem Cells" @default.
- W2384189192 hasPublicationYear "2008" @default.
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