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- W2384201644 abstract "Objective To study the effects of sCD40-EGFP modified dendritic cells(DC) on immunological function of experimental animals and to provide experimental basis for DCinduced dornor-specific immune torlerance.Methods Standard cloning techniques were employed to construct sCD40-EGFP fusion gene which was identified with double enzyme digestion and DNA sequencing.Then the recombinant plasmid was transfected into mouse dendritic cells with lipofectamine.sCD40-EGFP fusion protein secreted by DC in the culture supernatant was confirmed by fluorescence spectrophotometer and SDS-PAGE.Balb/c mice were sensitized with intraperitoneal injection of unmodified or modified dendritic cells.The mouse peripheral blood mononuclear cells(PBMC) as reaction cells were incubated with different groups of dendrite cells.MTS assay was used to detect lymphocytes proliferation.Lactate dehydroge-nase release method was used to examine the cytotoxic activity.Results The fusion gene was observed in dendritic cells and the fusion protein was detected in supernatant.sCD40-EGFP fusion protein was shown to have an evidenced inhibition on lymphocyte proliferation in response to unsensitized or sensitized Balb/c mice.Lymphocyte proliferation in different groups of mice induced by sCD40-EGFP modified dendritic cells were significant decreased.sCD40-EGFP fusion protein obviously inhibited the cytotoxic activity of lymphocytes,and the cytotoxic activity of lymphocytes of mice induced by sCD40-EGFP modified dendritic cells were significantly reduced.Conclusion Dendritic cells with stable expression of sCD40-EGFP fusion protein can obviously reduce lymphocyte proliferation in response to allogenic mice and reduce the cytotoxic activity of lymphocytes." @default.
- W2384201644 created "2016-06-24" @default.
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- W2384201644 date "2009-01-01" @default.
- W2384201644 modified "2023-09-25" @default.
- W2384201644 title "The effect of sCD40 modified dendritic cells on proliferation and cytotoxicity of lymphocytes in experimental animals" @default.
- W2384201644 hasPublicationYear "2009" @default.
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