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- W2384202461 abstract "【Objective】To investigate the role of the novel human C2H2-zinc finger gene ZNF580 in the proliferation and migration of human umbilical vein endothelial cells(HUVEC).【Methods】By the induction of liposome,the plasmids pEGFP-ZNF580 was transfected into EAhy926 cells with LipofectamineTM 2000 for 48 h.The localization was estimated by fluorescence microscope.The expression of ZNF580 was evaluated by RT-PCR and Western blotting.MTT colorimetric assay and cell migration assay were designed to investigate the effects of ZNF580 on endothelial cell growth and motility.【Results】The EA.hy926 cells were successfully transfected with plasmids pEGFP-ZNF580 transiently(the transfection efficiency rate was 60%).ZNF580 enhanced endothelial cell proliferation and migration by 3 fold and 2.5 fold,respectly.【Conclusion】These results elucidate the important role that ZNF580 may promote the migration and proliferation of endothelial cells,which provides a foundation for a novel approach to regulate angiogenesis." @default.
- W2384202461 created "2016-06-24" @default.
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- W2384202461 date "2011-01-01" @default.
- W2384202461 modified "2023-09-23" @default.
- W2384202461 title "Effect of ZNF580 gene on the proliferation and migration of human umbilical vein endothelial cells" @default.
- W2384202461 hasPublicationYear "2011" @default.
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