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- W2384207917 abstract "The aim of this study was to investigate the effect of supplementation of caffeine in freezing extender on frozen-thawed semen quality.Optimize freezing and thawing protocol in 0.5 mL straws.Supplementation of caffeine directly into preliminary design freezing extender,the concentration of caffeine was 0,0.2,0.4,0.6 mg/mL,determined the best concentration of caffeine.Then compared with the traditional TCG extender and the AndrohepCryoGuardTM extender,comparison of three different freezing curves on the frozen-thawed sperm quality.The frozen sperm samples were thawed by warming them at 37 ℃ water bath for 30 s and 50 ℃ water bath for 13 s.The results showed that there was a significantly higher percentage of progressive motility,membrane integrity and acrosome integrity in caffeine supplemented groups than control group(P0.05),the best concentration was 0.2 mg/mL.The preliminary design was better than the TCG(P0.05),but it had a significant variance with AndrohepCryoGuardTM extender(P0.05).Three different freezing curve had different influence on sperm quality parameters.Freezing curve A had a higher percentages of TMS,PMI and NAR of spermatozoa than B and C(P0.05),thawed at 50 ℃ for 13 s was better than 38 ℃ for 30 s." @default.
- W2384207917 created "2016-06-24" @default.
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- W2384207917 date "2010-01-01" @default.
- W2384207917 modified "2023-09-28" @default.
- W2384207917 title "Effects of Caffeine on the Cryopreservation of Boar Semen and Optimizationg of Freezing and Thawing Protocal of Rapid Freezing Techniques" @default.
- W2384207917 hasPublicationYear "2010" @default.
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