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- W2384223305 abstract "The radish self-incompatibility line Nau-DY06 was identified with the compatibility index and the fluorescence microscopy method.An ORF encoding 853 amino acids was amplified by RT-PCR from cDNA of the stigma of Nau-DY06 with the primer derived from the conserved sequences of the SRK genes in different crops.The gene was designated as RsSRK-a (GenBank accession number:GQ121139).Its identity with the SRK3 in Brassica rapa,the SRK13-b in B.oleracea var.acephala,was 89% respectively.The molecular weight of the predicated protein encoded was 96.6 × 103 and its theoretical pI was 6.69.Semi-quantitative RT-PCR analysis showed that expression of the RsSRK-a was detectable in the stigma of two days before anthesis and especially high at anthesis stage.Expression profiling of the RsSRK-a in radish stigma indicated that it might play an important role in the selfincompatibility.The results provide important information for the exploration of the mechanism and utilization of self-incompatibility in radish." @default.
- W2384223305 created "2016-06-24" @default.
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- W2384223305 date "2010-01-01" @default.
- W2384223305 modified "2023-09-23" @default.
- W2384223305 title "The full length cDNA cloning of RsSRK-a gene and its expression analysis in radish (Raphanus sativus L.)" @default.
- W2384223305 hasPublicationYear "2010" @default.
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