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- W2384226875 abstract "Objective: To study the effect of rosiglitazone on the ability of proliferation, conglutination and transfere of endothelial progenitor cell in vitro. Methods: Total mononuclear cells were isolated from the umbilical cord blood with ficoll density gradient centrifugation, after the redblood cells were precipitated by hydroxyethyl starch, and cultured in vitro. Cells were cultured for 7 days, which expressed CD34/CD133/VEGFR-2/VE-Cadherin tested by flow cytometry and ability of intaking DIL-ac-LDL and FITC-UEA-1 tested under fluorescence microscope were identified as EPCs. EPCs were determined after treatment with rosiglitazone (1, 5, 10 mmol/L) alone and in the presence of H2O2 (500 umol/L) for 24 h. EPCs' functions were assayed with CCK-8, Boyden cabin, and adherent cells were counted under inverted microscope. Results: Compared to the control group, rosiglitazone groups, concentration dependence improved cells' function(sP0.05), there was no significant difference between rosiglitazone 5 mmol/L group and 10 mmol/L grou(pP0.05). In the same versus to the H2O2 group rosiglitazone groups concentration-dependent protected EPCs from oxidative stress(P0.05). Conclusion: Rosiglitazone has other effect of anti-oxidative stress beside euglycemic agent on EPCs. Rosiglitazone concentration-dependent promotes EPCs' functions and improves the injury of oxidative stress caused by H2O2." @default.
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- W2384226875 date "2008-01-01" @default.
- W2384226875 modified "2023-09-25" @default.
- W2384226875 title "Effect of rosiglitazone on ability of proliferation, conglutination and transfere of endot-helial progenitor of cells in vitro" @default.
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