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- W2384237021 abstract "Objectives:To investigate persistent infection of Chlamydia pneumoniae(C.pneumoniae) in patients with respiratory tract infection and the value of blood based nested polymerase chain reaction(nPCR) in diagnosing persistent infection of C.pneumoniae. Methods:A prospective study for C.pneumoniae infectin was conducted in 137 patients with respiratory tract infection from August 2000 to February 2001,and 74 healthy volunteers as controls were also examined.The presence of infection was investigated by nPCR for C.pneumoniae DNA from peripheral blood mononuclear cells(PBMC) specimens and microimmunofluorescence (MIF) testing for C.pneumoniae specific IgG,IgM and IgA antibodies. Results:Prevalence of C.pneumoniae DNA in PBMC in patients with respiratory tract infection was higher than that of healthy volunteers(36.5% vs 12.2%, P 0.01).C.pneumoniae specific MIF serology confirmed exposure to the bacterium in 41(82%) of the 50 nPCR positive patients,and all of the nPCR positive volunteers were positive by C.pneumoniae specific serology.The nPCR positive rate was 55.2% in asthma patients,44.4% in acute bronchitis,39.4% in chronic obstructive pulmonary disease,33.3% in bronchiectasis,and 28.8% in pneumonia. Conclusions:C.pneumoniae may be hardored in human monocytes,and become persistent infection.The persistent infection of C.pneumoniae may be related with episodes of asthma,exacerbations of COPD,and it may be associated with pneumonia and bronchitis.Since conventional diagnostic methods are of limited value on identifying patients with persistent infection,detection of C.pneumoniae DNA in PBMC by nPCR may prove a useful means of diagnosing persistent infection of C.pneumoniae." @default.
- W2384237021 created "2016-06-24" @default.
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- W2384237021 date "2002-01-01" @default.
- W2384237021 modified "2023-09-23" @default.
- W2384237021 title "Clinical stydy on detection of chlamydia pneumoniae DNA in peripheral blood mononulcear cells by nested ploymerase chain reaction in patients with respiratory tract infection" @default.
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