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- W2384239093 abstract "Objective To investigate the results of several digestive methods in separating placenta-derived mesenchymal stem cells(PDMSCs) from rabbits' placenta.Methods Placentas were adoped from the pregnant rabbits model by uterine-incision delivery and were processed by three combinations of digestive juice mixed with collagenaseⅡ,collagenaseⅣ,dispase and DNaseⅠ:group A(collagenase Ⅳ + dispase + DNaseⅠ) ;group B(collsgenase Ⅳ + DNaseⅠ);group C(collagenase Ⅱ+DNaseⅠ).The isolated adherent cells were cultured and observed with inverted microscope day by day.The HE and alkali-phosphatase staining were used to observe the primary cell cultured.The viability and the doubling generation time were determined by staining with trypan blue.Results The step-by-step separation had less effect on cell vigoration.The adherent cells obtained from group C were more vigorous compared with group B and group A,and the statistical difference between each group was remarkable(P 0.05).The cells from group C did not express alkali-phosphatase,the morphous of those cells were regulated,but the alizarin Bordeaux staining was positive after inducing.The doubling generation time of the rabbit placenta-derived mesenchymal cells was 28 ～ 56 h.Conclusion The enzymatic digestion mixed with collagenase Ⅱ and DNase Ⅰ and step-by-step separation methods could effectively extract mesenchymal stem cells(MSCs) from the rabbits' placenta." @default.
- W2384239093 created "2016-06-24" @default.
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- W2384239093 date "2007-01-01" @default.
- W2384239093 modified "2023-09-26" @default.
- W2384239093 title "Comparison with methods in separating placenta-derived mesenchymal stem cells from rabbits' placenta" @default.
- W2384239093 hasPublicationYear "2007" @default.
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