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- W2384249144 abstract "Objective To establishe a nested-PCR method for direct detection of SFV proviral DNA in peripheral blood lymphocytes(PBLs)of rhesus monkey.Methods Amplification products of two pairs of primer of SFV-1 pol region from PBLs and RMK cells of rhesus monkeys were separated by 1% agarose gel electrophoresis.The cultured rhesus monkey kidney RMK cell line served as a positive control showing foamy cell pathology.The amplification products of positive control was confirmed by sequencing analysis.The cDNA of SRV-1 served as negative control.Result DNA of SFV provirus was detected directly from PBLs and cultured monkey kidney cells.The results of nested-PCR detection of PBLs and cytological changes of cultured kidney cells were identical with each other.Conclusion This method is a rapid,safe,efficient to detect SFV in rhesus monkeys.It can be an important molecular biological technique in detection of virus infection in rhesus monkeys,and may significantly improve the quality of laboratory monkeys for both researches and vaccine production." @default.
- W2384249144 created "2016-06-24" @default.
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- W2384249144 date "2009-01-01" @default.
- W2384249144 modified "2023-09-25" @default.
- W2384249144 title "Detection of Simian Foamy Virus(SFV)by Nested-PCR" @default.
- W2384249144 hasPublicationYear "2009" @default.
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