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- W2384253230 abstract "As a basic technique of modern molecular biology,molecular cloning has become an important tool for biomedical research. The traditional method for molecular cloning usually includes two key processes: restriction digestion and ligation. The success of a traditional cloning depends on suitable restriction sites and high ligation efficiency to a great degree. In the present study,we have developed a DNA cloning method mediated by exonuclease Ⅲ. Because this method is based on the 3' to 5' exonuclease activity of exonuclease Ⅲ and the intrinsic DNA repair machinery of bacteria,and is independent of DNA ligase,we designated it as ligation-independent cloning( LIC). We optimized the reaction parameters including the quantity of enzyme for each reaction,reaction time,reaction temperature and the ratio of vector to fragment,and found that this method showed high efficiency and reliability under optimal condition. We have thus established a rapid,simple and efficient DNA cloning method." @default.
- W2384253230 created "2016-06-24" @default.
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- W2384253230 date "2016-01-01" @default.
- W2384253230 modified "2023-09-26" @default.
- W2384253230 title "Exonuclease III-mediated DNA Molecular Cloning" @default.
- W2384253230 hasPublicationYear "2016" @default.
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