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- W2384257091 abstract "AIM: To establish a simple,sensitive method of high performance liquid chromatography tandem mass spectrometry(HPLC-MS/MS) for the determination of Indapamide in human plasma.METHODS: Repaglinide calcium was used as internal standard.Indapamide was separated on an Agilent-ZORBAX-C18 column(2.1 mm×150 mm,5.0 μm).The mobile phase consisted of acetonitrile-0.1% formic acid(90∶10).Electrospray ionization(ES1) was applied and operated in the positive multiple reaction monitoring(MRM) mode,used the transitions of +m/z 388.4 m/z 247.4 and +m/z 384.2 m/z 231.3 to quantify Indapamide and the internal standard respectively.RESULTS:The linear calibration curve was obtained over the concentration range of 0.5-50 ng/mL.The limit of quantification was 0.5 ng/mL.The inter-and intra-day precision(RSD) were less than 15%.The average recovery was between 94.60% and 110.33%.CONCLUSION: The method had been proved to be specific,convenient,sensitive and suitable for the clinical investigation of Indapamide pharmacokinetics." @default.
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- W2384257091 date "2012-01-01" @default.
- W2384257091 modified "2023-09-27" @default.
- W2384257091 title "Quantitative determination of Indapamide in human plasma by high performance liquid chromatography-tandem mass spectrometry" @default.
- W2384257091 hasPublicationYear "2012" @default.
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