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- W2384293386 abstract "Objective To measure telomere length by hybridization with biotin-labeled probe and study its application in forensic practice.Methods Genomic DNA was digested with restriction enzyme Rsa I and Hinf I,the products were separated by 0.8% agarose gel electrophoresis followed by Southern blotting with 5’terminal labeled oligonucleotide(TTAGGG)3 probe and chemiluminescence detection. The mean telomere length was calculated by comparing with standard kilobase sized marker.Results The hybridization bands were showed by stringently controlling the transfer condition,the probe working concentration and hybridization temperature(55 ℃).Conclusion Hybridization with biotin-labeled probe gives strong hybridization signal and is non-radioactive,and is reliable and stable to determinate telomere length." @default.
- W2384293386 created "2016-06-24" @default.
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- W2384293386 date "2009-01-01" @default.
- W2384293386 modified "2023-09-25" @default.
- W2384293386 title "A Biotin-Labeled Probe to Measure Telomere Length in Peripheral Leukocytes" @default.
- W2384293386 hasPublicationYear "2009" @default.
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