Matches in SemOpenAlex for { <https://semopenalex.org/work/W2384293616> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2384293616 abstract "Objective To investigate whether human umbilical cord blood mesenchymal stem cells would be able to differentiate into hepatocyte - like cells in vitro, and explore the mechanism of inducing differentiation.Methods Human umbilical cord blood samples were obtained sterilely from healthy parturients. MNCs were isolated by lymphocyte separation medi-um(density 1 .077g/ml) , MSCs were obtained by adhere culture. The surface antigen expression of MSCs were detected by flow cytometry. MSCs were induced with DMEM medium,containing 2%FBS, 1×ITS,and HGF alone, FGF4 alone, HGF + FGF4 or no growth factor respectively. The markers of hepatic linear cells, C - met and FGFR2 were examined before induction , and after 7, 14, 21 and 28 day induction by methods of RT - PCR, immunocytochemistry and PAS in every group . Results MSCs expressed CD29 and CD44 strongly, but did not express CD34. AFPmRNA and protein were detected on day 7 after induction. Albumin mRNA and protein, CK18 ,hepatocyte antigen, glycogen were detected on day 21 after induction . The expression of C-met and FGFR2 increased with induce culture.The markers of hepatocyte linear did not appear without growth factor. The positive percent of every hepatocytic marker at the same time point had statistically difference between HGF + FGF4 and HGF alone or FGF4 alone( P 0 .05) . Conclusion Both HGF and FGF4 can induce umbilical cord blood MSC to differentiate into hepatocyte - like cells singly or coordinately. There may be positive regulation mechanism between growth factor and its corresponding receptors." @default.
- W2384293616 created "2016-06-24" @default.
- W2384293616 creator A5086934712 @default.
- W2384293616 date "2004-01-01" @default.
- W2384293616 modified "2023-09-23" @default.
- W2384293616 title "Inducing differentiation of human umbilical cord blood mesenchymal stem cells into hepatocyte-like cells in vitro" @default.
- W2384293616 hasPublicationYear "2004" @default.
- W2384293616 type Work @default.
- W2384293616 sameAs 2384293616 @default.
- W2384293616 citedByCount "0" @default.
- W2384293616 crossrefType "journal-article" @default.
- W2384293616 hasAuthorship W2384293616A5086934712 @default.
- W2384293616 hasConcept C100787162 @default.
- W2384293616 hasConcept C10205521 @default.
- W2384293616 hasConcept C123012128 @default.
- W2384293616 hasConcept C153911025 @default.
- W2384293616 hasConcept C16685009 @default.
- W2384293616 hasConcept C170493617 @default.
- W2384293616 hasConcept C198826908 @default.
- W2384293616 hasConcept C202751555 @default.
- W2384293616 hasConcept C203014093 @default.
- W2384293616 hasConcept C20875687 @default.
- W2384293616 hasConcept C2776955114 @default.
- W2384293616 hasConcept C2776996007 @default.
- W2384293616 hasConcept C2780467284 @default.
- W2384293616 hasConcept C2780932548 @default.
- W2384293616 hasConcept C28328180 @default.
- W2384293616 hasConcept C553184892 @default.
- W2384293616 hasConcept C55493867 @default.
- W2384293616 hasConcept C71924100 @default.
- W2384293616 hasConcept C86803240 @default.
- W2384293616 hasConcept C95444343 @default.
- W2384293616 hasConceptScore W2384293616C100787162 @default.
- W2384293616 hasConceptScore W2384293616C10205521 @default.
- W2384293616 hasConceptScore W2384293616C123012128 @default.
- W2384293616 hasConceptScore W2384293616C153911025 @default.
- W2384293616 hasConceptScore W2384293616C16685009 @default.
- W2384293616 hasConceptScore W2384293616C170493617 @default.
- W2384293616 hasConceptScore W2384293616C198826908 @default.
- W2384293616 hasConceptScore W2384293616C202751555 @default.
- W2384293616 hasConceptScore W2384293616C203014093 @default.
- W2384293616 hasConceptScore W2384293616C20875687 @default.
- W2384293616 hasConceptScore W2384293616C2776955114 @default.
- W2384293616 hasConceptScore W2384293616C2776996007 @default.
- W2384293616 hasConceptScore W2384293616C2780467284 @default.
- W2384293616 hasConceptScore W2384293616C2780932548 @default.
- W2384293616 hasConceptScore W2384293616C28328180 @default.
- W2384293616 hasConceptScore W2384293616C553184892 @default.
- W2384293616 hasConceptScore W2384293616C55493867 @default.
- W2384293616 hasConceptScore W2384293616C71924100 @default.
- W2384293616 hasConceptScore W2384293616C86803240 @default.
- W2384293616 hasConceptScore W2384293616C95444343 @default.
- W2384293616 hasOpenAccess W2384293616 @default.
- W2384293616 hasRelatedWork W1970349351 @default.
- W2384293616 hasRelatedWork W2023875843 @default.
- W2384293616 hasRelatedWork W2048095173 @default.
- W2384293616 hasRelatedWork W2347600349 @default.
- W2384293616 hasRelatedWork W2350275792 @default.
- W2384293616 hasRelatedWork W2354663024 @default.
- W2384293616 hasRelatedWork W2358691146 @default.
- W2384293616 hasRelatedWork W2359293853 @default.
- W2384293616 hasRelatedWork W2367979327 @default.
- W2384293616 hasRelatedWork W2368611012 @default.
- W2384293616 hasRelatedWork W2370350167 @default.
- W2384293616 hasRelatedWork W2376082197 @default.
- W2384293616 hasRelatedWork W2385347306 @default.
- W2384293616 hasRelatedWork W2387497206 @default.
- W2384293616 hasRelatedWork W2392554996 @default.
- W2384293616 hasRelatedWork W2400305972 @default.
- W2384293616 hasRelatedWork W2440869274 @default.
- W2384293616 hasRelatedWork W2758579584 @default.
- W2384293616 hasRelatedWork W3031480532 @default.
- W2384293616 hasRelatedWork W3145969997 @default.
- W2384293616 isParatext "false" @default.
- W2384293616 isRetracted "false" @default.
- W2384293616 magId "2384293616" @default.
- W2384293616 workType "article" @default.