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- W2384294088 abstract "Based on the enzymatic effect of lactate dehydrogenase(LDH) on the reaction of lactic acid and the oxidized form of nicotinamide adenine dinucleotide(NAD),the inhibition effect of metal ion on the enzymatic reaction was studied under optimum condition.The result showed that Cu2+ has significant inhibited effect on the enzymatic reaction of LDH.The inhibition constant(KQS) of Cu2+is 9.1 μmol/L and the inhibition is of non-competitive type.According to the Michaelis-Menten equation under the action of inhibitor,the enzymatic reaction can be used for the quantitative determination of copper ion.It was found also that EDTA can be used to eliminate the interference of a certain amount of Cu2+in the determination of the substrate with the enzymatic reaction of LDH." @default.
- W2384294088 created "2016-06-24" @default.
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- W2384294088 date "2008-01-01" @default.
- W2384294088 modified "2023-09-24" @default.
- W2384294088 title "Influence of Metal Ion Inhibitor on the Enzymatic Method" @default.
- W2384294088 hasPublicationYear "2008" @default.
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