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- W2384295054 abstract "Objective To clone and characterize a novel gene which might be involved in liver regeneration.Methods A 76 h regenerative liver tissue cDNA library was constructed by SMART technology.One EST from suppression subtractive hybridization was probed by Dig and carried out phage in situ hybridization to clone positive clone from 76h cDNA library;Construction of expressed vector was made and the specific expressed protein was purified,and its multiclone antibody was produced by injecting fusion protein products to rabbit.Dot blot technique monitored its mRNA variation during 0～76 h.Results A novel full-length gene whose function was unknown by BlAST examination was cloned successfully, and was called temporally liver regeneration related protein (LRRP) and deposited in GenBank (accession NO.AY098917). The results that blasted in RGD (rat genome database) showed that it was localized in 19 q12 and was composed of four extrons and three introns.Dot blot results indicated that its expression quantity increased obviously in 76 h after hepatectomy.Conclusion The cloning of LRRP gene and its multiclone antibody provides a good base for the studies of liver generation related protein." @default.
- W2384295054 created "2016-06-24" @default.
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- W2384295054 date "2005-01-01" @default.
- W2384295054 modified "2023-09-24" @default.
- W2384295054 title "The expression and analysis of the cloning of a up-regulated novel gene during liver regeneration in rat" @default.
- W2384295054 hasPublicationYear "2005" @default.
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