Matches in SemOpenAlex for { <https://semopenalex.org/work/W2384295953> ?p ?o ?g. }
Showing items 1 to 65 of
65
with 100 items per page.
- W2384295953 abstract "A putA gene was isolated from the genomic library of Sinorhizobium fredii HN01 by molecular and biotechnological methods,and it had 82% and 86% identities with the sequence of the proline dehydrogenase from S.meliloti 1021 at nucleotide and amico acid level,respectivly.With the suicide plasmid pK18mob,the polar mutant GXHNPB and non-polar mutant GXHNPA inactivated in putA gene were constructed through homologous recombination.The putA gene was cloned into pLAFRJ vetor resulting in a complemention plasmid pGXHN37.The complement strains GXHNPHA and GXHNPHB were constructed by transforming pGXH37 into host strains GXHNPA and GXHNPB,respectively.The results of growth test showed that there was no difference among HN01,mutant and complement strains when were cultivated on completemedium YMB,but the mutants showed no growth in the MM medium containing proline as the sole carbon and nitrogen source.Plant tests revealed that mutants had lower nodulation efficiency ability and nitrogen-fixation,and nodulated 1 day later on soybean compared with the wild-strain HN01." @default.
- W2384295953 created "2016-06-24" @default.
- W2384295953 creator A5047100596 @default.
- W2384295953 date "2010-01-01" @default.
- W2384295953 modified "2023-09-27" @default.
- W2384295953 title "Cloning of the putA gene from Sinorhizobium fredii HN01" @default.
- W2384295953 hasPublicationYear "2010" @default.
- W2384295953 type Work @default.
- W2384295953 sameAs 2384295953 @default.
- W2384295953 citedByCount "0" @default.
- W2384295953 crossrefType "journal-article" @default.
- W2384295953 hasAuthorship W2384295953A5047100596 @default.
- W2384295953 hasConcept C104317684 @default.
- W2384295953 hasConcept C121050878 @default.
- W2384295953 hasConcept C143065580 @default.
- W2384295953 hasConcept C187882448 @default.
- W2384295953 hasConcept C19924922 @default.
- W2384295953 hasConcept C199360897 @default.
- W2384295953 hasConcept C22744801 @default.
- W2384295953 hasConcept C2777304268 @default.
- W2384295953 hasConcept C2779815552 @default.
- W2384295953 hasConcept C41008148 @default.
- W2384295953 hasConcept C515207424 @default.
- W2384295953 hasConcept C54355233 @default.
- W2384295953 hasConcept C86803240 @default.
- W2384295953 hasConceptScore W2384295953C104317684 @default.
- W2384295953 hasConceptScore W2384295953C121050878 @default.
- W2384295953 hasConceptScore W2384295953C143065580 @default.
- W2384295953 hasConceptScore W2384295953C187882448 @default.
- W2384295953 hasConceptScore W2384295953C19924922 @default.
- W2384295953 hasConceptScore W2384295953C199360897 @default.
- W2384295953 hasConceptScore W2384295953C22744801 @default.
- W2384295953 hasConceptScore W2384295953C2777304268 @default.
- W2384295953 hasConceptScore W2384295953C2779815552 @default.
- W2384295953 hasConceptScore W2384295953C41008148 @default.
- W2384295953 hasConceptScore W2384295953C515207424 @default.
- W2384295953 hasConceptScore W2384295953C54355233 @default.
- W2384295953 hasConceptScore W2384295953C86803240 @default.
- W2384295953 hasLocation W23842959531 @default.
- W2384295953 hasOpenAccess W2384295953 @default.
- W2384295953 hasPrimaryLocation W23842959531 @default.
- W2384295953 hasRelatedWork W1484375858 @default.
- W2384295953 hasRelatedWork W1514442582 @default.
- W2384295953 hasRelatedWork W1581522956 @default.
- W2384295953 hasRelatedWork W1926019068 @default.
- W2384295953 hasRelatedWork W1970112925 @default.
- W2384295953 hasRelatedWork W1975289803 @default.
- W2384295953 hasRelatedWork W1975725700 @default.
- W2384295953 hasRelatedWork W1980401895 @default.
- W2384295953 hasRelatedWork W1985880986 @default.
- W2384295953 hasRelatedWork W2006696799 @default.
- W2384295953 hasRelatedWork W2010986867 @default.
- W2384295953 hasRelatedWork W2013124264 @default.
- W2384295953 hasRelatedWork W2030122978 @default.
- W2384295953 hasRelatedWork W2039303387 @default.
- W2384295953 hasRelatedWork W2042324049 @default.
- W2384295953 hasRelatedWork W2076754757 @default.
- W2384295953 hasRelatedWork W2105540614 @default.
- W2384295953 hasRelatedWork W2127252043 @default.
- W2384295953 hasRelatedWork W2462386517 @default.
- W2384295953 hasRelatedWork W5619332 @default.
- W2384295953 isParatext "false" @default.
- W2384295953 isRetracted "false" @default.
- W2384295953 magId "2384295953" @default.
- W2384295953 workType "article" @default.