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- W2384306557 abstract "Objective To induce the immunoregulatory cells in mixed lymphocytes reaction(MLR) by FTY720 in combination with costimulation blockades(anti-CD154 and anti-CD80 monoclonal antibodies,Cos.Blockades) in mice.Methods Cos.Blockades(10 μg/mL) and FTY720(0,100,200,400,800 and 1 600 ng/mL) were added into MLR consisting of the splenocytes from BALB/c and C57BL/6J.The lymphocyte proliferation and CD4+CD25+ regulatory T-cells(Treg) proportion were measured by MTT assay and FACS,respectively.The induced cells were cultured with the same number of C57BL/6J splenocytes,either under the presence of exogenous IL-2 or not,to observe the reversibility of anergic state.Cells harvested from the primary MLR were added into the second MLR to test the antigen-specificity,and the 615 mouse splenocytes were served as the third-party. Results The lymphocyte proliferation and CD4+CD25+ Treg proportion in the FTY720+Cos.Blockades groups were increased in a dose-dependent manner.When the FTY720 concentration was 1 600 ng/mL,there was significant difference in the lymphocyte proliferation in the primary MLR between FTY720+ Cos.Blockades group and Cos.Blockade group(0.299 vs 0.391,P0.05).And when the FTY720 concentration was 800 and 1 600 ng/mL,there were significant differences of CD4+CD25+ Treg proportion between FTY720+Cos.Blockades group and Cos.Blockades group(5.44% vs 3.47%,and 6.91% vs 3.47%,respectively,P0.05).When the FTY720 concentration was 1 600 ng/mL,the cells induced either in FTY720+Cos.Blockades group or in Cos.Blockades group had the characteristics of anergic cells.However,this was not found in those with FTY 720 alone.The immunoregulatory cells induced in MLR manifested the ability to suppress the secondary MLR antigen specifically. Conclusion FTY720 and Cos.Blockades can synergistically induce and expand the immunoregulatory cells in vitro,and these cells may down-regulate the immune response in an antigen-specific manner." @default.
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- W2384306557 date "2008-01-01" @default.
- W2384306557 modified "2023-09-26" @default.
- W2384306557 title "Immunoregulatory cells induced in vitro by FTY720 in combination with costimulation blockades" @default.
- W2384306557 hasPublicationYear "2008" @default.
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