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- W2384328647 abstract "Objective:To establish a sensitive and rapid HPLC-MS/MS method for the determination of 5-hydroxymethyl furfural in Chinese traditional medicine injection.Methods:The HPLC separation was performed on Zorbax SB-C18(150 mm × 2.1 mm,5 μm)with methanol added 0.1% formic acid solvent mixture solution(20∶ 80) as the mobile phase,the flow rate was 0.2 mL·min-1,column temperature was room temperature.Electrospray ionization(ESI)source was used.Quantization was performed by using selected multiple reaction monitoring(MRM)mode.Results: The results showed the linear range of 5-hydroxymethyl furfural was 0.05-20.0 μg·mL-1(r=0.9993),the limit of quantization was 10.0 ng·mL-1,the average recovery rate(n=9)was 99.2%,the RSD(n=5)of precision test and reproducibility test were 1.9% and 1.8%.Conclusion: The method is sensitive,rapid and accurate with good repeatability,which can be used for determination of the content and the quality control of 5-hydroxymethyl furfural in Chinese traditional medicine injection." @default.
- W2384328647 created "2016-06-24" @default.
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- W2384328647 date "2010-01-01" @default.
- W2384328647 modified "2023-09-26" @default.
- W2384328647 title "HPLC-MS/MS determination of the content of 5-hydroxymethyl furfural in Chinese traditional medicine injection" @default.
- W2384328647 hasPublicationYear "2010" @default.
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