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- W2384328648 abstract "OBJECTIVE:To investigate the inhibitory effect of heparanase antisense oligodeoxynucleotide (AS-ODN) on the invasiveness of human liver cancer cell line BEL-7402 in vitro. METHODS: AS-ODN complementary to the start codon region of heparanase mRNA respectively was designed and synthesized. The ODNs with the final concentration of 0 nmol/L, 100 nmol/L, 200 nmol/L, 300 nmol/L were delivered into BEL-7402 cells by Oligofectamine~ TM Reagent. After transfection the heparanase gene and protein expression level were evaluated by the reverse transcription polymerase chain reaction (RT-PCR) and Western blot respectively and the cell invasiveness was measured by the Matrigel invasion assay. RESULTS: The heparanase gene expression in 0, 100, 200, 300 nmol/L AS-ODN transfected groups were 0.911, 0.898, 0.774, 0.280,respectively;65×10~3 Hpa protein expressions were 0.923, 0.875, 0.834, 0.237 respectively;50×10~3 Hpa protein expressions were 1.872, 1.919, 0.821, 0.325,respectively. The heparanase gene expression, protein expression in 300 nmol/L AS-ODN transfected group decreased compared with the control group. Invasion cells in 0, 100, 200, 300 nmol/L AS-ODN transfected groups were 137±15, 141± 13, 134±18, 49±7,respectively. Invasion cells in 300 nmol/L AS-ODN transfected group decreased significantly compared with the normal control group,P=0.000. CONCLUSIONS: Heparanase AS-ODN complementary to the start codon region could significantly inhibit the invasiveness of human liver cancer cell line BEL-7402 by down regulating heparanase expression.[" @default.
- W2384328648 created "2016-06-24" @default.
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- W2384328648 date "2006-01-01" @default.
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- W2384328648 title "Inhibitory effect of heparanase antisense oligodeoxynucleotides on invasiveness ofBEL-7402 cell line" @default.
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