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- W2384351685 abstract "Objective To observe the effect of heat shock factor 1 (HSF1) on heat stress-induced apoptosis in Raw264.7 macrophages. Methods Raw264.7 cells transfected with pcDNA3.1 and pcDNA3.1-HSF1 were exposed to heat stress (42.5 degrees C +/- 0.5 degrees C) for 1 h and recovered at 37 degrees C for 6, 9, 12, and 24 h respectively. Flow cytometry (FCM), Hoechst 33258 staining and assays were performed to assess the apoptosis. Results After heat stress, FCM showed that apoptotic cells were increased significantly and reached the peak at 9 h in Raw 264.7 cells transfected with pcDNA3.1, and were characterized with classical morphologic changes including apoptotic body and nuclear condensation. Agarose gel electrophoresis showed that DNA ladder could be observed clearly at 6, 9, and 12 h after the heat stress. But the overexpression of HSF1 could reduce the number of apoptotic cells and inhibit fragmentation. Conclusion HSF1 can inhibit heat stress-induced apoptosis in Raw264.7 macrophages." @default.
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- W2384351685 date "2006-04-01" @default.
- W2384351685 modified "2023-09-23" @default.
- W2384351685 title "HSF1 inhibits heat stress-induced apoptosis in Raw264.7 macrophages" @default.
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