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- W2384365090 abstract "In the process of L-lactic acid fermentation by Bacillus coagulans using glucose as the feedstock,the yield of L-lactic acid could be increased through adding cellulases.To investigate the reason,a stable system of spectrophotometric determination of β-glucan substance was established to measure the content of β-glucan substance in the L-lactic acid fermentation broth,using carboxymethyl cellulose(CMC) as the standard substance of β-glucan.It was illustrated that the potential influence might be omitted when the concentration of sodium ions in 0.025～0.175 mol/L,calcium ions in 0.001～0.005 mol/L,and the concentration of proteins in 0～40 mg/L.The optimal condition for determination by Congo red method is the maximum absorption wavelength 550 nm,pH 8.0 with phosphate buffer and the reaction time 15 minutes.The experiments reveal that the content of β-glucan substance in L-lactic acid fermentation broth is rather low,and the reason why cellulases could enhance the yield of L-lactic acid needs further investigation." @default.
- W2384365090 created "2016-06-24" @default.
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- W2384365090 date "2011-01-01" @default.
- W2384365090 modified "2023-09-28" @default.
- W2384365090 title "Determination of β-glucan substances in L-lactic acid fermentation broth by Congo red method" @default.
- W2384365090 hasPublicationYear "2011" @default.
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