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- W2384369912 abstract "The primary structure of winter-wheat-bran antifreeze protein(TaAFP) is the foundation of its higher structure and functionality.Hence,the primary structure of TaAFP was determined by the combination of N-terminal sequencing and mass fingerprint overlapping analysis.The molecular weight of TaAFP was 13637.711 Da determined by MALDI-TOF-MS analysis,similar to that from SDS-PAGE analysis.TaAFP was cleaved by trypsin,hydroxylamine,and chymotrypsin,respectively.The cleaved peptides were further analyzed and connected to acquire its primary structure as MARKVIALAFLLLLTISLSKSNAARVKYNGGESGGGGGGGGGGGGGGNGSGSGSGYGYNYGKGGGQSGGGQGGGGGGGGGGGSNGSGSGSGYGYGYGQGNGGAQGQGSGGGGGGGGGGGGGGSGQGSGSGYGYGYGKGGGGGGGGGGDGGGGGGGGSAYVGRHE,with overlap rate of 100 %.Homogeneity analysis showed TaAFP was related with the cellular wall and cold resistance." @default.
- W2384369912 created "2016-06-24" @default.
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- W2384369912 date "2009-01-01" @default.
- W2384369912 modified "2023-09-25" @default.
- W2384369912 title "Determination of Primary Structure of Winter-Wheat-Bran Antifreeze Protein" @default.
- W2384369912 hasPublicationYear "2009" @default.
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