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- W2384390943 abstract "Objective To determine the 16S rDNA sequences of 36 strains of Halomonas sp.isolated from the seawater of the East China Sea and to study their bioactivities.Methods The 16S rDNA sequences of all isolated strains were amplified by PCR,and the obtained sequences were subjected to phylogenetic analyses using MEGA4.1 and multiple sequence alignment with Clustal X1.8 software.MTT assay was applied to assess the cytotoxic activities of their fermentation broth;ABTS and DPPH antioxidant models were applied to test the antioxidation activities of their fermentation broth.The HLE inhibition activities of the fermentation broth were tested by a stable HLE inhibitor screening model.Results Analysis of 16S rDNA sequences showed that the 36 strains had a high affinity(96%-99%) to the Halomonas sp.Fermentation broth of the 36 strains showed different degrees of cytotoxic activities,antioxidation activities,and HLE inhibition activities.Eleven strains inhibited the growth of HepG2 cells,with the inhibition rate ranging(4.40±1.2)% to(24.90±3.5)%;20 strains inhibited the growth of HL-60 cells,with the inhibition rate ranging(1.70±1.1)% to(50.90±4.2)%;7 strains showed ABTS radical scavenging capacity,with the scavenging rate ranging(4.49±2.1)% to(58.43±4.4)%;3 strains showed DPPH radical scavenging capacity,with the scavenging rate ranging(5.68±3.7)% to(59.06±3.2)%,and 3 strains showed HLE inhibition activities,with the inhibition rate ranging(12.71±1.81)% to(71.19±5.62)%.Conclusion All the 36 Halomonas sp.strains show different degrees of cytotoxic effects,antioxidation activities,and HLE inhibition capacities.Several strains with high activities possess the potential for medicinal application." @default.
- W2384390943 created "2016-06-24" @default.
- W2384390943 creator A5066870388 @default.
- W2384390943 date "2011-01-01" @default.
- W2384390943 modified "2023-09-23" @default.
- W2384390943 title "Identification and bioactivities analysis of Halomonas sp.from seawater" @default.
- W2384390943 hasPublicationYear "2011" @default.
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