Matches in SemOpenAlex for { <https://semopenalex.org/work/W2384396215> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W2384396215 abstract "To clone aldehyde dehydrogenase gene aldC from Bacillus halodurans XJU-1 and express aldC fusion proteins with a His6-tag,aldC gene without terminator was amplified by PCR and cloned into plasmid pET30b(+).The recombinant plasmid was transformed into E.coli BL21(DE3).The transformant colony was then induced with IPTG and expressed a fusion proteins with 6×His at C terminus.The target protein was analysed by SDS-PAGE and Km values was investigated.The gene aldC was amplified and cloned into plasmid pET30b(+) successfully.The molecular weight of the fusion enzyme as estimated by SDS-PAGE was approximately 56,000.The enzyme activity was 3.5 times than the contrast bacteria.The Km values of the enzyme were 2.874mM for acetaldehyde.The recombinant plasmid was successfully constructed and the aldehyde dehydrogenase gene was expressed with His6 tag in E.coli BL21(DE3)." @default.
- W2384396215 created "2016-06-24" @default.
- W2384396215 creator A5008348071 @default.
- W2384396215 date "2012-01-01" @default.
- W2384396215 modified "2023-09-25" @default.
- W2384396215 title "Cloning of Aldehyde Dehydrogenase Gene aldC and Expression of Bacillus halodurans XJU-1" @default.
- W2384396215 hasPublicationYear "2012" @default.
- W2384396215 type Work @default.
- W2384396215 sameAs 2384396215 @default.
- W2384396215 citedByCount "0" @default.
- W2384396215 crossrefType "journal-article" @default.
- W2384396215 hasAuthorship W2384396215A5008348071 @default.
- W2384396215 hasConcept C104317684 @default.
- W2384396215 hasConcept C121050878 @default.
- W2384396215 hasConcept C123894998 @default.
- W2384396215 hasConcept C150194340 @default.
- W2384396215 hasConcept C153911025 @default.
- W2384396215 hasConcept C170344550 @default.
- W2384396215 hasConcept C19924922 @default.
- W2384396215 hasConcept C199360897 @default.
- W2384396215 hasConcept C22744801 @default.
- W2384396215 hasConcept C25747580 @default.
- W2384396215 hasConcept C40767141 @default.
- W2384396215 hasConcept C41008148 @default.
- W2384396215 hasConcept C55493867 @default.
- W2384396215 hasConcept C86803240 @default.
- W2384396215 hasConceptScore W2384396215C104317684 @default.
- W2384396215 hasConceptScore W2384396215C121050878 @default.
- W2384396215 hasConceptScore W2384396215C123894998 @default.
- W2384396215 hasConceptScore W2384396215C150194340 @default.
- W2384396215 hasConceptScore W2384396215C153911025 @default.
- W2384396215 hasConceptScore W2384396215C170344550 @default.
- W2384396215 hasConceptScore W2384396215C19924922 @default.
- W2384396215 hasConceptScore W2384396215C199360897 @default.
- W2384396215 hasConceptScore W2384396215C22744801 @default.
- W2384396215 hasConceptScore W2384396215C25747580 @default.
- W2384396215 hasConceptScore W2384396215C40767141 @default.
- W2384396215 hasConceptScore W2384396215C41008148 @default.
- W2384396215 hasConceptScore W2384396215C55493867 @default.
- W2384396215 hasConceptScore W2384396215C86803240 @default.
- W2384396215 hasLocation W23843962151 @default.
- W2384396215 hasOpenAccess W2384396215 @default.
- W2384396215 hasPrimaryLocation W23843962151 @default.
- W2384396215 hasRelatedWork W1989220160 @default.
- W2384396215 hasRelatedWork W2037293097 @default.
- W2384396215 hasRelatedWork W2047580767 @default.
- W2384396215 hasRelatedWork W2061032979 @default.
- W2384396215 hasRelatedWork W2070967071 @default.
- W2384396215 hasRelatedWork W2183466625 @default.
- W2384396215 hasRelatedWork W2340171010 @default.
- W2384396215 hasRelatedWork W2347736790 @default.
- W2384396215 hasRelatedWork W2352464428 @default.
- W2384396215 hasRelatedWork W2358542590 @default.
- W2384396215 hasRelatedWork W2368683082 @default.
- W2384396215 hasRelatedWork W2369123771 @default.
- W2384396215 hasRelatedWork W2371571139 @default.
- W2384396215 hasRelatedWork W2375999777 @default.
- W2384396215 hasRelatedWork W2377924023 @default.
- W2384396215 hasRelatedWork W2378519304 @default.
- W2384396215 hasRelatedWork W2393946924 @default.
- W2384396215 hasRelatedWork W2760543875 @default.
- W2384396215 hasRelatedWork W2865318892 @default.
- W2384396215 hasRelatedWork W3118565808 @default.
- W2384396215 isParatext "false" @default.
- W2384396215 isRetracted "false" @default.
- W2384396215 magId "2384396215" @default.
- W2384396215 workType "article" @default.