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- W2384397741 abstract "Objective To investigate the effect of all-trans retinoid acid(ATRA) on myocardial ischemia-reperfusion(IR) injury and explore the related mechanism. Methods H9c2 myocardial cells were pretreated with ATRA for 24 h and then subjected to hypoxia/re-oxygenation injury(6-hour hypoxia and 1-hour re-oxygenation). ATRA's effect on cell viability in H9c2 cells was measured using a water soluble tetrazolium salt-1(WST-1) assay, and cell apoptosis was quantified by annexin Ⅴ-fluorescein isothiocyanate(FITC) based flow cytometry. Apoptosis-related activated caspase-3,Bcl-2 and mitogen-activated protein kinase(MAPKs) signaling were measured by Western blotting. Results WST-1 assay revealed greater cell viability in ATRA 0.01 μmol/L and 1 μmol/L treated group(P 0.01). Flow-cytometry analysis demonstrated ATRA's(1 μmol/L) anti-apoptotic effect on IR in H9c2 cells(5.8%±0.5% vs 2.6%±0.2%, P0.01). Western blotting revealed that compared with controls, ATRA pre-treatment increased Bcl-2 expression(0.78±0.01 vs 1.08±0.02,P0.01), reduced activated caspase-3(1.24±0.03 vs 0.97±0.04, P0.01) and phosphorylation of p38(1.28±0.18 vs 0.81±0.08), c-Jun N-terminal kinases(JNK)(1.21±0.01 vs 0.93±0.03), and extracellular signal-regulated kinases(ERK)(1.19±0.02 vs 0.93±0.02)(all P0.01). Conclusions Our results showed that ATRA effectively increased H9c2 cell survival rate and reduced H9c2 cell apoptosis after I/R in vitro. This anti-apoptotic effect might be associated with down-regulation of MAPKs signaling." @default.
- W2384397741 created "2016-06-24" @default.
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- W2384397741 date "2014-01-01" @default.
- W2384397741 modified "2023-09-23" @default.
- W2384397741 title "All-trans retinoid acid protects against myocardial ischemia reperfusion injury via inhibiting apoptosis signaling" @default.
- W2384397741 hasPublicationYear "2014" @default.
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