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- W2384398547 abstract "To research the key genes in glycerol synthesis mechanism, Candida glycerinogenesis,a safety industrial yeast strains,harboring the perfect ability to yield high production of food-grade glycerol,was used to obtain the key gene.In order to further increasing production and reducing by-products,the research of the key genes in glycerol synthesis mechanism is the necessary.In this study, CgGPD,encoding the key enzyme glycerol 3-phosphate dehydrogenase,was cloned,using the genome of C.glycerinogenesis template. The serial analysis of gene expression with ScGPP2 from Saccharomyces cerevisiae bytac promoter in Escherichia coli.After 48hfermentation,the specific activity of CgGPD reached 60mU/mg and the production of glycerol reached 2.52g/L under 30g/L glucose.The study showed that CgGPDplays a key role in the synthesis of glycerol,and over-expression of CgGPD is beneficial to glycerol synthesis.The results provide a theoretical basis for metabolic regulation by over-expression of CgGPD to improved production of process food-grade glycerol and reduce the difficulty of downstream separation and purification,to further increase the molecular level of food-grade glycerol production." @default.
- W2384398547 created "2016-06-24" @default.
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- W2384398547 date "2014-01-01" @default.
- W2384398547 modified "2023-09-27" @default.
- W2384398547 title "Study on key glycerol synthesis genes from Candida glycerinogenesis to production food grade glycerol" @default.
- W2384398547 hasPublicationYear "2014" @default.
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