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- W2384456450 abstract "It had been proved that the change of ALP activity had obvious effect on the morphology of osteocyte and digestive function in the metamorphism stage of Japanese flounder(Scophthatmus maximus) larvae.In order to study the expression of alkaline phosphatase(ALP) gene and the effects of triiodothyronine(T3) on the expression of ALP gene in S.maximus,real-time fluorescent quantitative PCR was applied to detect the expression of ALP mRNA in eight different tissues which were intestine,muscle,spleen,heart,gill,liver,kidney,brain and the S maximus' kidney cell(SMKC) treated by T3 in five different concentrations(0 nmol/L,50 nmol/L,75 nmol/L,100 nmol/L,200 nmol/L).The results showed that the expression of ALP mRNA were various in different tissues,and was tissue-specific.The expression of ALP mRNA was the highest in heart,followed by liver and gill,and the lowest was in muscle.There was significant difference on expression of alkaline phosphatase(ALP)gene in SMKC after treated by different concentrations of T3.From the results,it can be concluded that ALP gene was expressed in eight different tissues,but there was significant difference in expression level,with tissue-specific characters.The expression of ALP mRNA had an incremental trend with the increase of T3.It was indicated that expression of ALP gene was positively regulated by T3,and was concentration-dependent." @default.
- W2384456450 created "2016-06-24" @default.
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- W2384456450 date "2011-01-01" @default.
- W2384456450 modified "2023-09-25" @default.
- W2384456450 title "Expression of alkaline phosphatase(ALP) gene in tissues of Scophtha-tmus maximus and relationship between thyroid hormone and alkaline phosphatase" @default.
- W2384456450 hasPublicationYear "2011" @default.
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